I put the saline mixture in a sterile glass container. I tried it right away. Then I put that container in the refrigerator. I plan on taking it out in the morning and nuke it. Then let it cool before using. When I poured the solution in the nebulizer I put too much in…had to empty one. I need to get something to measure the amount. I do 10 minutes with my nebulizer and just quit nebulizing when got to that time.