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@peabody88

Update to all those glued to this riveting discussion -- I'm now further befuddled. My repeat Quantiferon Gold came back negative, which isn't CRAZY surprising, but what seems odd to me is the huge (at least it seems so to me) shift in the value just a month apart:

8/24/18 - Positive* at 1.06 iU/mL; NIL was 0.01 (IU/mL); TB-NIL was 1.07 (IU/mL); Mitogen-NIL was >10 (IU/mL).
9/19/18 - Negative* at 0.13 iU/mL; NIL was 0.01 (IU/mL); TB-NIL was 0.14 (IU/mL); Mitogen-NIL was >10 (IU/mL).

From the preliminary research I've done, you can revert from positive to negative or convert from negative to positive in a month, particularly when it's a low positive (I was just slightly above the low positive maximum value of 1 on first testing), but it's usually just a small change -- like 0.01-0.05 points. My chest X-ray and CT scan were both normal, and while I did initially have a slight mucus-producing cough, it's since disappeared and my only "potential" TB symptoms would be unexplained fever (but it's low-grade) and night sweats (but it's not like sheet-drenching; in fact, it's almost more feeling hot/flushed and emitting body odor from the armpits although the rest of the body remains dry) or possibly a genitourinary form of extrapulmonary TB manifesting with urinary frequency, so I am by no means convinced I do (or do not) have TB. My questions are thus:

1. IS that amount of variation within a month normal, or does it more suggest some sort of disruptive influence (see question 2) or mishandling/testing by the lab?
2. What factors can affect the Quantiferon Gold result (specific medications, autoimmune diseases, abnormal levels of something else, etc.)? Are there any other diseases/conditions that are cross-reactive that the initial positive might have been reacting to?
3. What is the potential significance (if any) to the fact that the first (positive) test was conducted two weeks after treatment with Doxycycline antibiotic, and five days after treatment with two doses of Diflucan for a suspected vaginal yeast infection (culture later negative), but the second (negative) test was conducted two weeks after starting/while still taking another round of Doxycycline?
4. Again, anybody else had a false positive? Other than the fact that it was negative, which is more in line with our expectations since I don't travel much, is there any other reason to think the second test is more accurate than the first?

I've never had a positive TB skin or blood test previously, including my most recent tests in 2015 (skin) and 2011 (skin AND blood). Love to hear if anyone else has any insights/similar experience.

*For a test to be considered positive, the TB antigen tube value minus the Nil tube value must be ≥0.35 IU/mL (FDA cleared breakpoint). Low positive values (0.35–1.0 IU/mL) may not repeat as positive in some patient populations; some authorities recommend that the patient be retested to confirm low positive tests. My calculated value of 1.06 (TB antigen tube value minus the Nil tube value) can show a 52% reversion rate.

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Replies to "Update to all those glued to this riveting discussion -- I'm now further befuddled. My repeat..."

Have you received any more info regarding this? I am very confused since the same thing happened to me recently. My first test had a TB1-Nil of 0.59, TB2-Nil of 0.02, and Mitogen-Nil of 9.93. I got a chest x-ray afterwards, which came out fine, and I had no symptoms, so I retook the test at a different lab, and my results were negative: TB1-Nil of 0.07, TB2-Nil of 0.09, Nil value of 0.01, and Mitogen value of >10. As you can see, this is a big variation. Also, my last test from a year ago was negative. Upon researching the test, I do see that the lab is supposed to receive the sample within 14 hours of collection. My first test results show that the lab did not receive my sample for over 24 hours. I wonder if this is what affected my results. Also, the first lab took only 1 vial of blood, and the second lab took 4 vials (which upon reading is the correct way). I want to make sure that I am truly negative and not missing anything. I am also curious to see if there are other conditions that can possibly cause a false positive so I can get checked out for those too.