Was your endometrial cancer tested for mutations in the gene POLE?

Many thanks for responding so quickly and for the info. Was this at Mayo?

I'm interested in it for the prognostic value. (I do not have colorectal cancer in my family.) I will feel quite differently about further treatment depending on the result.

I also think it is a reasonable thing to want because the most recent version of the NCCN Guidelines for endometrial carcinoma recommends molecular classification based on 3 tests: POLE sequencing, immunohistochemistry (IHC) for mismatch repair (MMR) proteins, and IHC for p53 (ENDO-A p. 3 of 4). This is based on a number of publications over the last few years that categorize endometrial carcinomas into 4 groups: 1) POLE mutants (from sequencing), 2) mismatch repair defective (from MMR IHC), 3) copy number high (from p53 IHC), and 4) copy number low (everything else). POLE mutants have a very good prognosis, while copy number high have the worst prognosis, and includes some high grade endometriod carcinomas as well as serous carcinomas.

Many POLE mutant cancers are like mine, presenting with high grade and greater depth of myometrial invasion. But some copy number high cancers are also like mine. I will feel quite differently about how aggressively my cancer should be treated if it's POLE mutant vs. copy number high. (IHC for MMR and p53 have been done on my specimen.) Once I'm given a treatment plan from my current team (hopefully soon), I'll have to decide whether to go looking for a second opinion, and whether I'll have to make access to a POLE test a criteria for where.

Hello NatureGirl15,
I am recently diagnosed with endometrial carcinoma 1B Grade 3. I had a total hysterectomy 2 weeks ago and await pelvic radiation therapy (28 doses).

Kindly post the link to the link to the NCCN Guidelines for endometrial carcinoma recommendation of molecular classification based on 3 tests. I would like to pursue this further with my physicians.

Wishing you all the very best.

Hello NatureGirl15,
I am recently diagnosed with endometrial carcinoma 1B Grade 3. I had a total hysterectomy 2 weeks ago and await pelvic radiation therapy (28 doses).

Kindly post the link to the link to the NCCN Guidelines for endometrial carcinoma recommendation of molecular classification based on 3 tests. I would like to pursue this further with my physicians.

Wishing you all the very best.

I'll try attaching the guidelines to this post, but that may not be successful.

The NCCN is annoying in that they make you register to get access to their most recent treatment guidelines. I used an account from my former job, but I think you should be able to register too. Go to https://www.nccn.org/guidelines/category_1. Select "Uterine Neoplasms". I can't see what it does at this point because it is automatically logging me in, but I think it will give you the opportunity to register or log in. You probably need to say that you're a healthcare provider or medical professional.

I realized a number of things after I started this thread. One is that POLE mutation testing is most definitely NOT standard of care for endometrial cancer in the US, and almost no hospitals offer it to newly diagnosed patients. You can sort of figure out why from the NCCN guidelines: although they recommend POLE testing, the treatment algorithms don't include the results at all. This contrasts with the European guidelines (https://ijgc.bmj.com/content/31/1/12.long), which recommend considering no treatment for patients with POLE mutations. No US physician is going to recommend foregoing treatment because of this mutation without it being in a set of official guidelines. So if you want to use this in making a decision, the doctor is going to argue against you.

The immunohistochemistry for mismatch repair (MMR) genes and for p53 should be on your pathology report. If there is an MMR defect, then there's probably no point in looking at POLE. Being "positive" for p53 doesn't rule out a "good" POLE mutation, but probably makes it less likely.

I'm happy that I spent $450 out of pocket to get the POLE test done at Stanford, even though I didn't get the result I wanted. I'm not completely sure what I would have done if it had been positive, but I definitely would not be having chemo. Since then, I have also had a multi-gene sequencing panel done on my tumor, because I'm a molecular nerd, but if you want to do that, be very careful about what they are going to try to charge you, if/when insurance doesn't cover it. (The list price at my hospital is well over $10,000. But after investigating, I'm getting it for less than $1000.)

Hi,
I've just gotten a 2nd opinion surgical path report consult. I don't understand some of it and am hoping that you can shed some light. What is MMR proficient mean? What is BRAF positive mean? Thanks so much.

I did manage to get a POLE mutation test on my tumor by having it sent to Stanford ($450 as of Sept. 2022; I didn't mess with trying to get insurance to pay for it). This is the only place in the country that I could find that is advertising a single gene test for POLE and is interested in taking outside specimens.

However, most academic medical centers sequence POLE as part of a "next-generation sequencing panel", where they sequence dozens to a few hundred different genes in a single test. I would have rather had a sequencing panel done, but it took a long, educational investigation to figure out how much this would cost if insurance wouldn't pay for it (which they almost certainly wouldn't at my stage. Insurance is more likely to pay for a sequencing panel for advanced or recurrent cancers such as @mimito6's. I know this kind of thing because I'm a molecular biologist who used to work in a lab that did these panel tests.).

The hospital where I'm being treated has a "price transparency" spreadsheet that lists the cash price for such a panel at well above $10,000. I contacted the lab (which you're not supposed to do as a patient, but I was super-frustrated), and eventually they got back to me and said that I could self-pay for the panel for less than $1000 if the correct procedure was followed. So much for price transparency.

Anyway, my POLE test was negative, which is not the good outcome, so now I have to decide what to do.

I'm interested in what people think. My tumor is a Stage 1B, FIGO grade 3 endometrioid endometrial cancer. My current Dr. has recommended 3-6 cycles of chemo plus vaginal brachytherapy. I'm scheduled to start chemo next week. My insurance won't cover a second opinion at any of the very highly rated non-local hospitals, so I got a second opinion at the second large local hospital system, and they recommended pelvic radiation therapy; no chemo. I have an appointment for a 3rd opinion at a cancer center belonging to a third local hospital system next week, less than 24 hours before I'm scheduled to start chemo. I'm planning to decide for certain what to do after that, but am currently thinking that I will go ahead and start chemo.

What do you think has fewer long-term side effects? Chemo or pelvic radiation? I'll put up with the short term side effects, but would rather not be dealing with peripheral neuropathy, lymphedema, urinary, bowel problems, etc. for the rest of my life.

Hi,
I was just wondering if anyone could explain the POLE testing and the effect in endometrial cancer? I was tested and found to be POLE negative, which is my understanding not the outcome you want. Does it effect the prognosis? Or recurrence?
I’d so appreciate any insight. I’ve read quite a bit, but it’s so technical. Thanks so much!

Hi,
I was just wondering if anyone could explain the POLE testing and the effect in endometrial cancer? I was tested and found to be POLE negative, which is my understanding not the outcome you want. Does it effect the prognosis? Or recurrence?
I’d so appreciate any insight. I’ve read quite a bit, but it’s so technical. Thanks so much!