I nebulize the albuterol and saline twice a day and do the Amikacin inhalation treatment once a day. I do the albuterol and saline at 4:00 a.m. then go back to sleep until 6 LOL! I take the nebulizer to work and do the next one around 4 in the afternoon. I just wash the pieces in hot soapy water. Then before bed I do the Amikacin treatment which is 10 x more time consuming with how you have to wash and sterilize each time. But it works and the more you do it; the quicker it goes. Totally worth it.