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@rlpostrp

Sorry, but your description of needle biopsy slide preparation is 100 % WRONG
.
It is done with this method :

"Prostate needle biopsy slides are prepared by fixing tissue cores in formalin, embedding them in paraffin wax, and slicing them into 4–5
m sections using a microtome. These thin sections are mounted on glass slides, stained (usually with hematoxylin and eosin), and examined microscopically by a pathologist to detect cancer".

So, it is NOT centrifuged by any means. The WHOLE sections are placed on a microscope slide and examined as a tissue , not "whatever sticks on a slide" after centrifuge.

Since the whole long section is examined (12 or more of them), pathologist can see not only the type of cells but how they are arranged and what gleason dominates and in what %.

If needle passes by EPE and catches it - it will be seen on a slide. When it passes neural pathways it will catch that part and pathologist will see if cancer is present around nerve ends. If it catches tissue that has IDC present, than ducts with infiltrated cancer cells will be seen.

So needle biopsy is not sometimes wrong because it can not see things, but because needle biopsy examines only about 1 % of the WHOLE gland so if it misses on its path the worst lesion, the result will not be 100 % correct.

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Replies to "@rlpostrp Sorry, but your description of needle biopsy slide preparation is 100 % WRONG . It..."

@surftohealth88 Thanks for your input. You are not entirely correct or incorrect. Some pathologists want their small volume biopsy core samples processed that way using paraffin blocks. The analogy offered here explains the inefficiency, and the need for a very patient histotech/cytotech and pathologist. The paraffin blocks from the cassette tray gismos are about 20mm (3/4") square. Imagine placing a few bread crumbs in the 3/4" x 3/4" paraffin block vs a small piece of bread. They can find the small amount of bread crumbs...but it takes longer, but it doesn't take too much more time, but it is a waste of all of the paraffin and minimal space occupied by the small sample volume in the block. So...
I encourage you to type into your browser search bar: "Is a cytocentrifuge used in processing prostate needle biopsy tissue for microscopic examination?" I did...Here is the "copy/paste" answer with focus on the key words like "concentration" and "monolayer":
"Yes, a cytocentrifuge is used to prepare prostate cells for microscopy. This technique allows for the 'concentration' of cells onto a microscope slide, creating a 'monolayer' that facilitates examination. The cytocentrifuge uses centrifugal force to deposit cells from a fluid sample onto the slide, which is particularly useful for analyzing prostate specimens in clinical settings.
How it Works:
A funnel assembly is attached to the slide, lined with filter paper to absorb excess fluid.
A small volume of the sample is placed in the funnel, and the centrifuge spins, depositing the cells onto the slide in a concentrated manner."

While some (a lot?) of pathologists might use the paraffin block method on the very small sample size from the needle biopsy, "all" of the Labs in which I was a Director for my career, used the cytocentrifugation method. Use of paraffin tissue blocks was reserved for actual "pieces" of post-surgical tissue. The procedure you describe is what happens more often to the tissue once the prostate is removed, and - per my first comment - sees the pathologist or histotech - visually examine ("gross") the prostate and dissect/sample the most suspicious/diseased parts of the prostate, then...as I mentioned as "a few other steps in between," the tissue is processed over night, then is placed in the cassette trays ("gismos") for the hot paraffin wax...the the blocks with the chunk of tissue are section in micro-thin sections on the microtome. Those sections are mounted on slides with he paraffin being melted away. The slides are then stained and examined.
Unfortunately, the term "biopsy" and post-prostatectomy tissue processing have been blurred. There have been a lot of guys on this blog who talk about the "biopsy" of their prostate "after" it has been removed. That is technically incorrect use of the term "biopsy." "Biopsies" occur before surgical removal from the body. The more correct term for the post-prostatectomy handling of the prostate would be: "gross histopathological handling and processing of the prostate, and examination of microtome-sectioned, stained tissue on microscope slides by a pathologist." So...
You're right...some people do it the way you searched and found, but most don't in my experience in California hospital-based Clinical and Anatomical Laboratory services. I appreciate your passion.
Thanks for the commentary.