@topf I think it can go bad but Dr told me the trimix goes bad quickly. It has to be kept refrigerated. Someone on this site recommend freezing. So I make up 10-15 shots and then freeze them and the vial. Bimix may have a shelf life but doesnt need refrigerated. Freezing would probably help. I have had my Trimix refrigerated for 7 months. Still working. Someone had said they tried the trimix without keeping refrigerated and still worked. So I tried one injection 4 days without refrigeration and it worked the same. The other two chemicals must do the job. Also vial is open? you mean the metal tab and needle marks? I think its still pretty sealed that way.