@sueinmn Thanks Sue, I feel it is more effective to use two vials of saline to help me clear the mucus though I dont have a lot of mucus. But I trust you, and will reduce the quantity to one vial. In addition, it looks like many BE patient has a genetic variant in CF test. I have one single variant which is found in 13% of CF Asian patients. I guess this genetic variant, though will not cause CF, but may be related to BE. That is to say, BE is a genetic disease and may cause frequent lung infection. Am I correct?