that first time i took it and had that 'miracle' experience i had taken a single 500 mcg capsule. after that, i took 500 mcg three times a day for several years. now i take 500 mcg twice a day, morning and evening. i have the MTHFR gene, and it helps enormously. an hour after 500 mcg molybdenum in the evening, i take one tablespoon of activated charcoal at least 3 days a week.