My biopsy was not a big deal at all. They had a giant scary needle for the numbing - I joked they should take that out on Halloween - but there was very little pain through the whole procedure. They use a gun-like device to shoot in and retrieve samples. You will have bloody ejaculate for about a month or so. Definitely a mood killer. All that I've read says that a biopsy is the ONLY way to diagnose cancer. All of the other methods are best guesses. Even a biopsy can miss cancer tissue, but if it finds cancer, you have cancer.