I have read that inflammatory chemicals are released in the body at around 4 am. Here is a quote from Google:

"according to research, a significant portion of cytokine release, particularly pro-inflammatory cytokines, tends to peak during the early morning hours, often around 4am, as the body transitions from sleep to wakefulness, aligning with the circadian rhythm and immune system activity during this time.

Key points about cytokine release and the circadian rhythm:
Peak activity during sleep:
While the body releases cytokines throughout the sleep cycle, the highest levels are often observed during the later stages of sleep and the early morning hours.

Morning stiffness:
This phenomenon is partly attributed to the increased release of inflammatory cytokines like TNF-alpha and IL-6 during the early morning, which can contribute to joint stiffness in individuals with conditions like arthritis.

Importance of sleep quality:
Adequate sleep is crucial for proper immune function, as disruptions in sleep patterns can affect the normal circadian rhythm of cytokine release. "

I am an early riser, so I take the prednisone really early, around 4 am. So far I haven't had any problems with it not lasting a full 24 hours.