I have many underlying gut problems in addition to SIBO, as I had 6 weeks of pelvic and abdominal radiation treatment 25 years ago and I also have a ton of scar tissue in my guts from several surgeries to reverse bowel obstructions but I don't think it matters if you take it the exact same time every day. It's not a laxative, just a softener so it's more mild. I take my 3 doses 3-4 hours apart from each other and I also take prescription Motegrity and an herbal prokinetic motility supplement called Iberogast. I think you could experiment with different times of the day to see what works best for you. For me, I notice that my constipation gets worse the later in the day, after I've started eating so I'll take more towards the end of the day since I generally wake up with a flat stomach and easier bowel movements after fasting overnight.